50:92–98ĭöring AS, Petersen M (2014) Production of caffeic, chlorogenic and rosmarinic acids in plants and suspension cultures of Glechoma hederacea. Acta Physiol Plant 38:76Ĭai X, Kang XY (2014) Plant regeneration from cell suspension-derived protoplasts of Populus × beijingensis. planifolia constitute the bases of future studies and above all a better biotechnological use of this crop.īarbasz A, Kreczmer B, Oćwieja M (2016) Effects of exposure of callus cells of two wheat varieties to silver nanoparticles and silver salt (AgNO 3). The establishment and characterization of cell suspension cultures of V. The growth kinetics of the cell suspension culture revealed a maximum cell growth (exponential growth phase) at day 16 and an 80% cell viability. The optimal condition found for biomass growth in suspension cultures was 0.5 g of inoculum density (fresh weight) in MS liquid, supplemented with 8.88 µM BA. Cultures were incubated under photoperiod at continuous stirring at 120 rpm on an orbital shaker. The effect of benzyladenine (BA) in different concentrations was evaluated. For that, friable calluses were obtained from immature seeds cultivated in MS medium supplemented with 0.45 µM thidiazuron (TDZ). The objective of this study was to establish and characterization of cell suspension cultures of V. The establishment and characterization of cell suspension cultures are an in vitro culture technique very useful for various plant biotechnological applications (production of secondary metabolites, mass micropropagation, protoplast isolation and fusion, gene transfer and the investigation of cell pathways).
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